Aliasger K Salem

Aliasger K Salem

, PhD
  • Professor, Chemical and Biomedical Engineering
  • Professor, Holden Comprehensive Cancer Center
  • Division Head, Pharmaceutics and Translational Therapeutics
  • Lyle and Sharon Bighley Endowed Professor in Pharmaceutical Sciences, Department of Pharmaceutical Sciences and Experimental Therapeutics / Division of Pharmaceutics and Translational Therapeutics

Research Narrative

Dr. Salem's research interests are primarily focused on self-assembling systems, the rational design of novel drug and gene delivery systems and on the development of sophisticated scaffolds for tissue-specific regeneration. In tissue engineering, Dr. Salem's laboratory applies microfabrication techniques to novel biomaterials to provide spatial control over tissue formation and to integrate minimally invasive scaffold delivery strategies. In drug/gene delivery, he is currently exploring the synergistic application of degradable particle technology, CpG oligonucleotides and heat shock proteins for generating sustained immunotherapeutic responses against cancer.

Curriculum vitae

Education
1998 BSc (1st Class Hons Applied Chemistry); Aston University, U.K.
2002 PhD (Pharmaceutical Sciences); University of Nottingham, U.K.
2002-2004 Postdoctoral Fellowship (Medicine); The Johns Hopkins University

Professional Experience
2014 - present Professor, College of Pharmacy, University of Iowa
2009-2014 Associate Professor, College of Pharmacy, University of Iowa
2004-2009 Assistant Professor, College of Pharmacy, University of Iowa
2002-2004 Postdoctoral Fellow, Department of Biomedical Engineering, School of Medicine, The Johns Hopkins University
2001-2002 Scientific Consultant, Regentec Ltd, Nottingham, U.K.
1998-2001 Scientist, Pharmaceutical Profiles, Nottingham, U.K.

Research Interests
Dr. Salem's research interests are primarily focused on self-assembling systems, the rational design of novel drug and gene delivery systems and on the development of vaccines that stimulate potent antigen-specific immune responses.  Dr Salem's laboratory applies microfabrication techniques to develop novel drug and gene delivery devices and to optimize control over polymer-cell interactions. The group is currently exploring the synergistic application of polymer particle technology,  CpG oligonucleotides, adenoviruses and heat shock protein therapy for generating sustained stronger immune responses against tumors.

Recent Publications

Joshi VB, Geary SM, Gross BP, Wongrakpanich A, Norian LA, Salem AK. Tumor lysate-loaded biodegradable microparticles as cancer vaccines. Expert Rev Vaccines, 2013.

Elangovan S, D'Mello SR, Hong L, Ross RD, Allamargot C, Dawson DV, Stanford CM, Johnson GK, Sumner DR, Salem AK. The enhancement of bone regeneration by gene activated matrix encoding for platelet derived growth factor. Biomaterials. 2014;35(2):737-47.

Worthington KL, Adamcakova-Dodd A, Wongrakpanich A, Mudunkotuwa IA, Mapuskar KA, Joshi VB, Allan Guymon C, Spitz DR, Grassian VH, Thorne PS, Salem AK. Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung. Nanotechnology. 2013;24(39):395101.

Joshi VB, Geary SM, Salem AK. Biodegradable particles as vaccine antigen delivery systems for stimulating cellular immune responses. Hum Vaccin Immunother. 2013;9(12).

Wei N, Yu Y, Joshi V, Schmidt T, Qian F, Salem AK, Stanford C, Hong L. Glucocorticoid receptor antagonist and siRNA prevent senescence of human bone marrow mesenchymal stromal cells in vitro. Cell Tissue Res. 2013;354(2):461-70.

D'Mello SR, Yoo J, Bowden NB, Salem AK. Microparticles prepared from sulfenamide-based polymers. J Microencapsul. 2013 Jul 18. [Epub ahead of print].

Geary SM, Lemke CD, Lubaroff DM, Salem AK. The combination of a low-dose chemotherapeutic agent, 5-fluorouracil, and an adenoviral tumor vaccine has a synergistic benefit on survival in a tumor model system. PLoS One. 2013;8(6):e67904.

Geary SM, Salem AK. Prostate cancer vaccines: Update on clinical development. Oncoimmunology. 2013;2(5):e24523.

Geary SM, Lemke CD, Lubaroff DM, Salem AK. Proposed mechanisms of action for prostate cancer vaccines. Nat Rev Urol. 2013;10(3):149-60.

Krishnamurthy S, Behlke MA, Ramachandran S, Salem AK, McCray PB Jr, Davidson BL. Manipulation of Cell Physiology Enables Gene Silencing in Well-differentiated Airway Epithelia. Mol Ther Nucleic Acids. 2012;1:e41.

Lemke CD, Geary SM, Joshi VB, Salem AK. Antigen-coated poly α-hydroxy acid based microparticles for heterologous prime-boost adenovirus based vaccinations. Biomaterials. 2013;34(10):2524-9.

Joshi VB, Geary SM, Carrillo-Conde BR, Narasimhan B, Salem AK. Characterizing the antitumor response in mice treated with antigen-loaded polyanhydride microparticles. Acta Biomater. 2013;9(3):5583-9.

Joshi VB, Geary SM, Salem AK. Biodegradable particles as vaccine delivery systems: size matters. AAPS J. 2013;15(1):85-94.

Yoo J, Kuruvilla DJ, D'Mello SR, Salem AK, Bowden NB. New class of biodegradable polymers formed from reactions of an inorganic functional group. Macromolecules. 2012;45(5):2292-2300.

Yoo J, D'Mello SR, Graf T, Salem AK, Bowden NB. Synthesis of the first poly(diaminosulfide)s and an investigation of their applications as drug delivery vehicles. Macromolecules. 2012;45(2):688-697.

Rosevear HM, Krishnamachari Y, Ariza CA, Mallapragada SK, Salem AK, Griffith TS, De Young BR, Wald M. Effect of combined locally delivered growth factors and systemic sildenafil citrate on microrecanalization in biodegradable conduit for vas deferens reconstruction. Urology. 2012;79(4):967.e1-4.

Wongrakpanich A, Joshi VB, Salem AK. Poly(galactaramidoamine) is an efficient cationic polymeric non-viral vector with low cytotoxicity for transfecting human embryonic kidney (HEK293) and murine macrophage (RAW264.7) cells. Pharm Dev Technol. 2013;18(5):1255-8.

Salem AK, Patil SD, Burgess DJ. Recent progress in non-viral nucleic acids delivery. Int J Pharm. 2012;427(1):1-2.

Jiang D, Salem AK. Optimized dextran-polyethylenimine conjugates are efficient non-viral vectors with reduced cytotoxicity when used in serum containing environments. Int J Pharm. 2012;427(1):71-9

Hong L, Wei N, Joshi V, Yu Y, Kim N, Krishnamachari Y, Zhang Q, Salem AK. Effects of glucocorticoid receptor small interfering RNA delivered using poly lactic-co-glycolic acid microparticles on proliferation and differentiation capabilities of human mesenchymal stromal cells. Tissue Eng Part A. 2012;18(7-8):775-84.

Geary SM Lemke CD Lubaroff DM and Salem AK. Tumor immunotherapy using adenovirus vaccines in combination with intramoral doses of CpG ODN. Cancer Immunology. Immunotherapy. 2011

Lemke CD Graham JB Geary SM Zamba G Lubaroff DM and Salem AK. Chitosan is a surprising negative modulator of cytotoxic CD8+ T cell responses elicited by adenovirus cancer vaccines.Molecular Pharmaceutics 2011.

Kim N Jiang D Jacobi A Lennox KA Rose S Behlke MA and Salem AK. Synthesis and characterization of mannosylated pegylated polyethylenimine as a carrier for siRNA.International Journal of Pharmaceutics. 2012.

Santillan DA Rai KK Santillan MK Krishnamachari Y Hunter S and Salem AK. Efficacy of Polymeric Encapsulated C5a peptidase-based Group B Streptococci Vaccines in a Murine Model.American Journal of Obstetrics & Gynecology 2011.

Lemke CD Graham JB Lubaroff DM and Salem AK. Development of an MHC class I Ld-restricted PSA peptide-loaded tetramer for detection of PSA-specific CD8+ T cells in the mouse. Prostate Cancer and Prostatic Diseases, 2011.

Intra J Zhang XQ Williams RL Zhu X Sandler AD and Salem AK. Immunostimulatory sutures that treat local disease recurrence following primary tumor resection. Biomedical Materials, 2011.

Geary SM Lemke CD Krishnamachari Y and Salem AK. New nanobiotechnological strategies for the development of vectors for cancer vaccines.  Pharmaceutical Biotechnology 2nd Edition. Wiley-Blackwell, 2011.

Kuruvilla DJ and Salem AK. Nanotechnology and nanomedicine in diabetes: an overview. Nanotechnology and nanomedicine in diabetes. Science Publishers, 2011.

Hong L Krishnamachari Y Seabold D Joshi V Schneider G and Salem AK. Intracelluar release of 17-β estradiol from cationic polyamidoamine dendrimer surface modified poly lactic-co-glycolic acid microparticles improves osteogenic differentiation of human mesenchymal stromal cells. Tissue Engineering, 2011.

Krishnamachari Y Geary SM Lemke CD and Salem AK. Nanoparticle delivery systems in cancer vaccines. Pharmaceutical Research, 2011.

Intra J and Salem AK. Rational design, fabrication, characterization and in vitro testing of biodegradable microparticles that generate targeted and sustained transgene expression in HepG2 liver cells. Journal of Drug Targeting, 2011.

Intra J, Salem AK. Fabrication, characterization and in vitro evaluation of poly(D,L-lactide-co-glycolide) microparticles loaded with polyamidoamine-plasmid DNA dendriplexes for applications in nonviral gene delivery. J Pharm Sci. 2010;99(1):368-84.

Petushkov A Ndiege N Salem AK and Larsen SC. Toxicity of Silica Nanomaterials: Zeolites, Mesoporous Silica, and Amorphous Silica Nanoparticles. Advances in Molecular Toxicology Vol 4. Elsevier 2010.

Salem AK and Behlke M. RNA Interference: Application to Drug Discovery and Challenges to Pharmaceutical Development. siRNA Delivery Vehicles, John Wiley & Sons Inc, 2010.

Petushkov A., J. Intra, J.B. Graham, S.C. Larsen, Salem AK. Effect of Crystal Size and Surface Functionalization on the Cytotoxicity of Silicalite-1 Nanoparticles. Chemical Research in Toxicology. 2009.

Krishnamachari Y. and Salem AK. Innovative Strategies for Co-delivering Antigens and CpG Oligonucleotides. Advanced Drug Delivery Reviews. 61 (2009) 205-217

Salem AK and G.J. Weiner. CpG oligonucleotides as Immunotherapeutic Adjuvants: Innovative Applications and Delivery Strategies. Advanced Drug Delivery Reviews. 61 (2009) 193-194

Goforth R., Salem AK, X. Zhu, S. Miles, X.Q. Zhang, J. Lee, A.D. Sandler. Immune Stimulatory Antigen Loaded Particles Combined with Depletion of Regulatory T-cells Induce Potent Tumor Specific Immunity in a Mouse Model of Melanoma. Cancer Immunology, Immunotherapy 2009

Intra J., Salem AK. Characterization of the Transgene Expression Generated by Branched and Linear Polyethylenimine (PEI)-Plasmid DNA Nanoparticles In Vitro and After Intraperitoneal Injection In Vivo. Journal of Controlled Release. 2008

Pearce ME, Mai HQ, Lee N, Larsen SC, Salem AK. Silicalite nanoparticles that promote transgene expression. Nanotechnology 2008

Intra J, J.M Glasgow, H. Q. Mai, Salem AK. Pulsatile release of biomolecules from polydimethylsiloxane (PDMS) chips with hydrolytically degradable seals. Journal of Controlled Release 2008

Clapper JD, M.E. Pearce, C.A. Guymon, Salem AK. Biotinylated Biodegradable Nanotemplated Hydrogel Networks for Cell Interactive Applications. Biomacromolecules 2008

Krishnamachari Y., M.E. Pearce, Salem AK. Self-assembly of cell-microparticle hybrids. Advanced Materials. 2008

Zhang XQ, J. Intra, Salem AK. Comparative study of poly (lactic-co-glycolic acid)-poly ethyleneimine-plasmid DNA microparticles prepared using double emulsion methods. Journal of Microencapsulation. 2008 1-13

Salem AK. Frontiers in Drug Design and Discovery. Volume 3. Journal of Medicinal Chemistry. 2008

Krishnamachari Y, Salem AK. Nano-Bio Polymers and Composites. Invited chapter in "Nano-and Biocomposites". Taylor & Francis/CRC Press. Edited by Lau and Hussain. 2008

Glasgow J, Salem AK. Stem Cells and Cancer. Invited review for "Encyclopedia of Stem Cell Research". Sage Publications. Edited by Clive Niels Svendsen, Ph.D., Allison D. Ebert, Ph.D. and Geoffrey J. Golson, Ph.D. 2008

Pearce ME, Salem AK. Stem Cell Research in Iowa. Invited review for "Encyclopedia of Stem Cell Research". Sage Publications. Edited by Clive Niels Svendsen, Ph.D., Allison D. Ebert, Ph.D. and Geoffrey J. Golson, Ph.D. 2008

Zhang XQ, J. Intra, Salem AK. Conjugation of polyamidoamine dendrimers on biodegradable microparticles for non-viral gene delivery. Bioconjugate Chemistry. 2007 Nov-Dec;18(6):2068-76 

Abbas AO, M.D. Donovan, Salem AK. Formulating PLGA particles for plasmid DNA delivery. Journal of Pharmaceutical Sciences.  2007 DOI: 10.1002/jps.21215

Pearce ME, J.B. Melanko, Salem AK. Biomedical applications of multifunctional nanorods. Pharmaceutical Research. 2007 Dec; 24(12):2335-52

Zhang XQ, C.E. Dahle, G.J. Weiner and Salem AK. A comparative study of the antigen-specific immune response induced by co-delivery of CpG ODN and antigen using fusion molecules or biodegradable microparticles. Journal of Pharmaceutical  Sciences. 2007 Dec;96(12):3283-92

Zhang XQ, C.E. Dahle, N.K. Baman, N. Rich, G.J. Weiner and Salem AK. Potent antigen-specific immune responses stimulated by co-delivery of CpG ODN and antigens in degradable microparticles. Journal of Immunotherapy, 30 (5): 469-478 July/August 2007

Salem AK, M. Behlke. siRNA delivery vehicles. Invited Chapter in the Book entitled "RNA Interference: Application to Drug Discovery and Challenges to Pharmaceutical Development". John Wiley & Sons Inc. 2007 Edited by Teresa N. Faria, Siew Peng Ho, and Oliver Steinbach.

Melanko JB, M.E. Pearce, Salem AK. Nanotubes/nanorods/nanofibers/fullerenes for nanoscale drug delivery. Invited Chapter in the Book entitled "Nanotechnology in Drug Delivery". Springer Science/American Association for Pharmaceutical Scientists (AAPS) 2007. Edited by Melgardt M. de Villiers, and Glen S. Kwon. 

Salem AK, M.K. Salem, H.K. Salem, S. Tapya. New technologies and therapies for cancer. Invited review for the "Encyclopedia of Cancer and Society". Sage Publications 2007 . Edited by J. Geoffrey Golson and Graham A. Colditz.

Sinclair J, Salem AK. Rapid localized cell trapping on biodegradable polymers using cell surface derivatization and microfluidic networking. Biomaterials. 27 2090-2094 MAR 2006

Baman NK, G.B. Schneider, T.L. Terry, R. Zaharias, Salem AK. Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels. International Journal of Nanomedicine, 1 (2): APR 2006

Salem AK. Frontiers in Drug Design and Discovery. Volume 2. Journal of Medicinal Chemistry. 2006 Dec 14;49(25):7555-6.

Salem AK, C.F. Hung, T.W. Kim, T.C. Wu, P.C. Searson, K.W. Leong. Multi-component nanorods for vaccination applications. Nanotechnology. 4 (16): 484-487  APR 2005

Hultgren A, M. Tanase, E.J. Felton, K. Bhadriraju, Salem AK, C.S. Chen, D.H. Reich. Optimization of yield in magnetic cell separations using nickel nanowires of different lengths. Biotechnology Progress. (21): 509-515  APR 2005

Salem AK and K.W. Leong.  Polymer scaffolds for tissue engineering and gene delivery. Invited Chapter in the Book entitled "Scaffolding in Tissue Engineering". CRC Press 2005 Edited by: Jennifer Elisseeff and Peter X. Ma

Salem AK, M. Chen, J. Hayden, K.W. Leong, and P.C. Searson. Directed assembly of multi-segment Au/Pt/Au nanowires. Nanoletters. 4 (6): 1163-1165 JUN 2004

Salem AK, J. Chao, K.W. Leong, and P.C. Searson. Receptor mediated self-assembly of multi-component magnetic nanowires. Advanced Materials.  16 (3): 268+ FEB 3 2004

Salem AK, P.C. Searson and K.W. Leong. Multifunctional nanorods for gene delivery. Nature Materials.  Oct 2003, 2, 668-671

Sakhalkar HS, M.K. Dalal, Salem AK, R. Ansari, J. Fu, M.F. Kiani, J. Hanes, K.M. Shakesheff and D.J. Goetz. Leukocyte inspired therapeutic particles that selectively and avidly adhere to inflamed endothelium in vitro and in vivo.  Proceedings of the National Academy of Sciences. 100 (26): 15895-15900 DEC 23 2003

Salem AK, F.R.A.J. Rose, R. Oreffo, X. Yang, M.C. Davies, J.R. Mitchell, C.J. Roberts, S. Stolnik, S.J.B. Tendler, P.M. Williams and K.M. Shakesheff. Porous polymer and cell composites that self assemble in situ. Advanced Materials. 15 (3): 210-213 FEB 5 2003

Salem AK, R. Stevens, R.G. Pearson, M.C. Davies, S.J.B. Tendler and K.M. Shakesheff. Interactions of 3T3 fibroblasts and endothelial with defined pore features.  Journal of Biomedical Materials Research. 61 (2) 212-217 Aug 2002

Salem AK, S.M. Cannizzaro, M.C. Davies, S.J.B. Tendler, C.J. Roberts, P.M. Williams and K.M. Shakesheff. Synthesis and characterisation of a degradable biotinylated polylactic acid-polyethylene glycol copolymer. Biomacromolecules. Sum 2001, 2, 575-580